2006. Analysis of transcriptome in rice and Arabidopsis

2006. Analysis of transcriptome in rice and Arabidopsis

DOWNLOAD: PDF
SÐT: 0981800855 (A. LONG)
PRICE: 100.000 VND
EMAIL: FOODCROPS@GMAIL.COM

ABSTRACT

Transcriptional regulation is a fundamental issue in plant biology. With the availability of completed sequenced genomes and tools for large-scale analysis of gene transcription, such as DNA microarrays, the dynamics of transcriptomes and their developmental regulation are being revealed. In this dissertation, I focused my research primarily on genome-wide analysis of the transcriptome in rice and Arabidopsis, with special interest on light-regulation. First, I investigated blue light regulation of Arabidopsis transcription factor gene expression. Studies of various photoreceptor mutants revealed the predomiant role of cryptochromes in blue light control of genome transcription. Second, I compared light-regulated genome expression in rice and Arabidopsis and found a higher correlation of expression in light than in darkness. Organ-specific expression profiles provide evidence for divergent light effects in different organs with specific light-responsive promoter c/s-elements involved. Next, I used a tiling microarray to analyze the transcription of rice chromosome 4. This analysis provided expression support for most of the gene models, detected transcriptional activities in non-annotated regions, and identified correlation between transcriptional activity and chromosome organization. Further, I examined transcriptional activities of selected rice transposon-related genes. Expression was detected and actively transcribed clades were identified, supporting potential adapted host function or active expression (and possible transposition). Finally, I participated in the construction and analysis of RICEATLAS, a cellular transcriptional atlas of rice. My research significantly advanced our knowledge of the genome expression and their regulation in the two plant model species with their genome complete sequenced  .